Journal: Materials today. Bio

Article Title: Precise delivery of doxorubicin and imiquimod through pH-responsive tumor microenvironment-active targeting micelles for chemo- and immunotherapy.

doi: 10.1016/j.mtbio.2022.100482

Figure Lengend Snippet: Fig. 10. Immunostaining of tumor tissues in tumor-bearing mice after treatments. (A) Immunohistochemistry images of tumor sections stained with CD3, CD8, and TNF-α antibodies. The scale bar is 100 μm. (B) Immunofluorescence images of tumor tissues after treatments for 12 days. The scale bar is 50 μm. Blue fluorescence represents the cell nucleus stained with DAPI. Green fluorescence represents the iNOS stained with the iNOS antibody conjugated with FITC.

Article Snippet: After 30 min, the tissue slice was stained with diluted iNOS antibody (Miltenyi Biotec, catalog: 130-116-357) at 4 C overnight.

Techniques: Immunostaining, Immunohistochemistry, Staining

Journal: Journal of Tissue Engineering

Article Title: Formation of neutrophil extracellular traps in the early stages exacerbate the healing process by regulating macrophage polarization in Achilles tendon-bone injury

doi: 10.1177/20417314251348038

Figure Lengend Snippet: PAD4 inhibition reduces M1 macrophage polarization after tendon-bone injury. (a) Immunohistochemistry (IHC) analysis of CD68 in tendon-bone section from control, TI, and TI + PAD4 inhibitor groups. (b) Quantification of CD11c positive cells. n = 6 mice/group. (c) IHC analysis of CD11c in tendon section from control, TI, and TI + PAD4 inhibitor groups. (d) Quantitative analysis of CD11c positive cells in c. n = 6 mice/group. (e) Western blot of iNOS, TLR4 and IL-1β in tendon tissue from control, TI, and TI + PAD4 inhibitor groups. (f) Quantification of relative gray intensity/GAPDH in c. n = 3 mice/group. Data are presented as the mean ± SEM. Scale bar = 1 mm. Significance was examined by one-way ANOVA (b, d, and f). ** P < 0.01, **** p < 0.0001.

Article Snippet: For M1 macrophages detection, sections were incubated with CD68 (14-0681-82; Invitrogen), CD11c (14-0114-82, Invitrogen), and iNOS (MAB9502; R&D systems) antibodies.

Techniques: Inhibition, Immunohistochemistry, Control, Western Blot

Journal: Molecular medicine reports

Article Title: Beneficial effect of 20‑hydroxyecdysone exerted by modulating antioxidants and inflammatory cytokine levels in collagen‑induced arthritis: A model for rheumatoid arthritis.

doi: 10.3892/mmr.2017.7389

Figure Lengend Snippet: Figure 7. Effects of HES on the protein expression of iNOS and COX‑2 in joint tissue of experimental rats. β‑actin was used as internal standard. Data are presented as the mean ± standard deviation for 10 rats in each group. **P<0.01, CIA vs. control; *P<0.05 HES 10 vs. CIA; **P<0.01 HES 20 vs. CIA. L1, control group; L2 CIA‑induced group; L3, HES 10 group; L4, HES 20 group; CIA, collagen‑induced arthritis; HES, 20‑hydroxyecdysone; 10, 10 mg/kg HES; 20, 20 mg/kg HES; iNOS, inducible nitric oxide synthase; COX‑2, cyclooxygenase‑2.

Article Snippet: The membrane was blocked with a mixture of 5% skimmed milk and Tween-20 in Tris-phosphate buffer solution (TPBS) for 1 h at 37 ̊C, and incubated with the primary antibodies, mouse monoclonal anti-inducible nitric oxide synthase (iNOS; 2982) and anti-cycloxygenase-2 (COX-2; 4842; both 1:1,200; Cell Signaling Technology, Inc., Danvers, MA, USA) and β-actin (2791; 1:800; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.) for 10 h at 37 ̊C.

Techniques: Expressing, Standard Deviation, Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: Environment and Behavior: Neurochemical Effects of Different Diets in the Calf Brain

doi: 10.3390/ani9060358

Figure Lengend Snippet: Brightfield photomicrographs of coronal sections ( A – C ) and histogram ( D ) showing the distribution of neuronal nitric oxide synthase (nNOS) immunoreactivity in the prefrontal cortex of the control diet group ( A ), milk diet group ( B ), and wean diet group ( C ). Comparison among the three groups shows no significant difference in neuronal densities (number of somata/mm 2 ; A–D ). In every group, nitrergic cells are non-pyramidal neurons with angular-( E , F ) or fusiform-( G ) shaped cell body. Scale bars = 200 µm in ( C ) (applies to ( A – C )); 20 µm in ( G ) (applies to ( E – G )).

Article Snippet: Thereafter, the sections were incubated in a solution containing rabbit anti-oxytocin polyclonal antibody (diluted 1:100; code PA5-26701; ThermoFisher Scientific, Bannockburn, IL, USA) or rabbit anti-MOG polyclonal antibody (diluted 1:100; code PA5-19602; ThermoFisher Scientific, Bannockburn, IL, USA) or mouse anti-nNOS monoclonal antibody (dilute 1:500; code TA300971; OriGene Technologies, MD, USA), or rabbit anti-c-Fos monoclonal antibody (diluted 1:100; code MA5-15055; ThermoFisher Scientific, IL, USA), 0.3% Triton X-100, and 1% normal goat serum for 48 h at 4 °C.

Techniques: Control, Comparison

Journal: Mediators of Inflammation

Article Title: Leptin Inhibits the Proliferation of Vascular Smooth Muscle Cells Induced by Angiotensin II through Nitric Oxide-Dependent Mechanisms

doi: 10.1155/2010/105489

Figure Lengend Snippet: Effect of leptin activation of Akt and STAT3 and iNOS expression in aortic VSMCs. Bar graphs show the differences in the time course of Akt (a) and STAT3 (b) activation/phosphorylation as well as iNOS (c) protein expression in leptin (10 nmol/L)-stimulated aortic VSMCs from Wistar and SHR. Data are expressed as mean ± SEM ( n = 10). Differences between groups were analysed by two-way ANOVA. In case of interaction between factors (strain and time of leptin stimulation), differences between groups were analysed by one-way ANOVA followed by Dunnet's test * P < .05, ** P < .01 versus unstimulated cells.

Article Snippet: Blots were then incubated overnight at 4°C with rabbit polyclonal anti-Akt1, rabbit polyclonal anti-phospho-(Thr 308 )-Akt (Upstate, Lake Placid, NY, USA), rabbit polyclonal anti-STAT3, rabbit polyclonal anti-phospho-(Tyr 705 )-STAT3 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), mouse monoclonal anti-iNOS (BD Transduction Laboratories, San Jose, CA, USA), rabbit polyclonal anti Nox2/gp91phox (Abcam, Cambridge, UK), or murine monoclonal anti- β -actin (Sigma) antibodies.

Techniques: Activation Assay, Expressing